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Document 0263
DOCN M9650263
TI [Coagulase-negative staphylococci in normal and chronically inflamed
conjunctiva]
DT 9605
AU Grasbon T; Mino de Kaspar H; Klauss V; Augenklinik, Universitat M nchen.
SO Ophthalmologe. 1995 Dec;92(6):793-801. Unique Identifier : AIDSLINE
MED/96147371
AB This study examines the prevalence of coagulase-negative Staphylococcus
species in normal and mildly inflamed conjunctiva, their sensitivity to
antibiotics, and their relationship to the remaining flora. PATIENTS AND
METHODS: In 99 patients including 9 HIV-positives in an early stage of
the infection, 100 conjunctival swabs were taken and microbiologically
investigated for bacteria and fungi. Thirty-four were from healthy eyes.
40 were from patients with chronic (n = 28) and unspecific (n = 12)
conjunctivitis, 17 were from patients with a variety of outer
inflammatory ocular conditions, and 9 were from the HIV group from
uninfected (n = 6) and infected (n = 3) conjunctivae. Samples from each
patient were collected with three moistened cotton swabs and directly
inoculated onto five different agars, followed by immersion into three
specific culture broths. Staphylococci were identified species-wise, the
other microorganisms genus-wise. Sensitivity to a broad spectrum of
antibiotics was determined by agar diffusion tests. OUTCOME:
Staphylococci were found in 89%, which was the highest prevalence
genus-wise. Of those, coagulase-negative species accounted for 86%,
while coagulase-positive Staphylococcus aureus was isolated in 12% of
all swabs. In the 86 smears positive for coagulase-negative
staphylococci, 151 different strains were isolated. In these strains,
resistance to the 13 tested antibiotics varied from 0% for vancomycin to
66% for penicillin. Strains which were isolated from patients with
chronic conjunctivitis showed a greater range of resistance than those
from normal flora, with significant levels for ciprofloxacin, gentamycin
and kanamycin (Mann-Whitney) U-test: P < 0.05). All except six strains
of staphylococci were identified strains represented ten species, of
which Staphylococcus epidermidis was most prevalent (74%), but only made
up 70% of all isolated strains of the coagulase-negative staphylococci.
Staphylococcus aureus and gram-negative bacteria were found
significantly more commonly in patients which chronic conjunctivitis
than in healthy eyes, while coagulase-negative species of the
Micrococcaceae family were significantly more prevalent in the healthy
than in the chronically inflamed conjunctiva (chi-square: P < 0.05).
CONCLUSION: The conjunctiva can simultaneously host several stems of
coagulase-negative staphylococci, which differ in regard to species and
resistance to antibiotics. This variety might indicate a microbiological
balance of the conjunctiva and be reduced in chronic inflammatory
conditions. In patients with chronic conjunctivitis the risk for
multiresistant coagulase-negative staphylococci is increased.
DE AIDS-Related Opportunistic Infections/DIAGNOSIS/MICROBIOLOGY
Blepharitis/DIAGNOSIS/MICROBIOLOGY Chronic Disease
Conjunctiva/*MICROBIOLOGY Conjunctivitis, Bacterial/*MICROBIOLOGY
Culture Media Drug Resistance, Multiple English Abstract Female
Human Keratoconjunctivitis/DIAGNOSIS/MICROBIOLOGY Male Microbial
Sensitivity Tests Prospective Studies Reference Values Staphylococcal
Infections/DIAGNOSIS/*MICROBIOLOGY Staphylococcus/CLASSIFICATION/DRUG
EFFECTS/ISOLATION & PURIF Staphylococcus aureus/DRUG EFFECTS/ISOLATION
& PURIF Staphylococcus epidermidis/DRUG EFFECTS/ISOLATION & PURIF
Support, Non-U.S. Gov't JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).